Using new 64-bit SPCM data acquisition software, the DCS‑120 FLIM system from Becker and Hickl records images of the full field of view of a microscope lens at diffraction-limited resolution. Image formats of up to 2,048 x 2,048 pixels with 256 time channels per pixel can be used. Two such images are recorded simultaneously by the two parallel channels of the DCS‑120 system.
Megapixel FLIM is extremely useful for tissue imaging, and when FLIM data of a large number of cells have to be compared. FLIM data of all cells in the field of view are obtained simultaneously, and under perfectly identical experimental conditions.