Spatial Light Output Characteristics of Solid-State Light Engines
Spatial Light Output Characteristics of Solid-State Light Engines - A Lumencor White Paper
In light microscopy, it is obvious that information is only obtainable from the portion of the specimen that is illuminated. Furthermore, it is self-evident, that if the illumination is not spatially uniform, then that non-uniformity will bias the image data.
However, intentional biasing of the illumination field is foundational to many of the techniques for increasing the spatial resolution of fluorescence microscopy that have emerged in the past 25 years. This White Paper describes the spatial output characteristics of solid-state light sources and how they are propagated into imaging applications.
Who should read this White Paper?
This White Paper is essential reading for professionals in optical engineering, microscopy, and photonics research, as well as decision-makers in life sciences, materials science, and biotech industries. Ideal for R&D leaders, application engineers, and product developers in fields such as fluorescence microscopy, this paper delves into the critical role of solid-state light sources, especially in contexts requiring highly specific illumination control for advanced imaging applications.
What does this White Paper contain?
The White Paper provides an in-depth exploration of the spatial light output characteristics of solid-state light engines, including lasers and LEDs, and their unique applications in light microscopy. It offers a detailed comparison between LED and laser illumination methods, highlighting key metrics like spectral distribution, light output efficiency, and spatial distribution. Additionally, it covers the concept of étendue - a measure of light output capacity—and its role in optimising the efficacy of different light sources for various microscopy techniques. Practical examples and real-world data illustrate how illumination field uniformity impacts spatial resolution and image quality in applications ranging from widefield fluorescence microscopy to super-resolution imaging.
Why should you read this White Paper?
Advancements in microscopy and imaging techniques have made it increasingly important to select the right light source for each application. This paper outlines the benefits and limitations of solid-state light engines, helping readers understand how to leverage LEDs and lasers to maximise imaging resolution, efficiency, and precision.
Whether you’re developing new microscopy technologies or optimising current imaging systems, this comprehensive guide offers actionable insights and technical data critical to informed decision-making.
